Document 0153
 DOCN  M9490153
 TI    Physiological effects of HIV infection on human intestinal epithelial
       cells: an in vitro model for HIV enteropathy.
 DT    9411
 AU    Asmuth DM; Hammer SM; Wanke CA; Division of Infectious Diseases, New
       England Deaconess Hospital,; Boston, MA 02215.
 SO    AIDS. 1994 Feb;8(2):205-11. Unique Identifier : AIDSLINE MED/94318204
 AB    OBJECTIVES: To determine the role of direct infection of intestinal
       cells with HIV-1 in the pathogenesis of HIV-related enteropathy.
       METHODS: We infected HT-29-18-C1 intestinal cells with the IIIB strain
       of HIV and examined the physiologic effects of enterocyte function.
       Dipeptidase-IV, aminopeptidase-N, gamma glutamic transferase, and
       alkaline phosphatase were measured in HIV-infected and control cultures.
       The cellular second messengers intracellular calcium and cyclic
       adenosine monophosphate were also measured in infected and control
       cultures. RESULTS: A persistent infection was established for > 95 days
       with peak supernatant reverse transcriptase and HIV p24 antigen levels
       of 5.17 log10 c.p.m./ml and 45 ng/ml, respectively. Brush-border enzyme
       activity (nmol of product/min/mg protein) tended to be lower in infected
       cell cultures compared with controls early in infection (P < 0.02).
       Baseline second messenger concentrations were similar but infected
       cultures responded to stimulation with a calcium ionophore with an
       exaggerated increase in intracellular calcium (P = 0.03). CONCLUSIONS:
       These results suggest that absorptive and secretory function of
       enterocytes may be altered by direct HIV infection and that additional
       physiologic experiments with this in vitro model may lead to a better
       understanding of the clinical syndrome of HIV enteropathy.
 DE    Alkaline Phosphatase/METABOLISM  Aminopeptidases/METABOLISM  Antigens,
       CD4/BIOSYNTHESIS  Calcium/METABOLISM  Carcinoma/PATHOLOGY  Colonic
       Neoplasms/PATHOLOGY  Cyclic AMP/METABOLISM  Dipeptidases/METABOLISM
       Gamma-Glutamyltransferase/METABOLISM  Gastrointestinal
       Diseases/COMPLICATIONS/MICROBIOLOGY  Human  HIV Infections/COMPLICATIONS
       HIV-1/*PHYSIOLOGY/PATHOGENICITY  Intestinal
       Mucosa/*MICROBIOLOGY/ULTRASTRUCTURE  Microvilli/ENZYMOLOGY  Second
       Messenger Systems  Support, U.S. Gov't, Non-P.H.S.  Support, U.S. Gov't,
       P.H.S.  Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).